FAM50A and neoplasm: Fig 1C depicts the results for unpaired CRC samples, while Fig 1D depicts the results for paired CRC samples. Following the observation of high FAM50A expression levels in CRC from a public database, we confirmed its expression in CRC tissue using IHC staining. A total of 145 patients with CRC were studied, comprising 67 women and 78 men. The baseline characteristics of this CRC cohort are presented in Table 2. Consistent with results of the analysis of data from TCGA database, FAM50A expression levels were higher in tumor tissue than in the adjacent normal tissue (P <  0.05, Figs 1E and 1F).