To assess whether ERK5 modulates the expression of GLI proteins in melanoma cells, ERK5 was silenced using lentiviral vectors expressing shRNAs targeting human ERK5 (shERK5-1/shERK5-2) in A375 and SK-Mel-5 cells (Fig. 4 and Fig. S2A). The gene discussed is MAPK7; the disease is melanoma.