SMOX and infection: (D) Wild-type BMDM cells were primed overnight with 1 μg/ml Pam3CSK4 and incubated with ΔRetS PAO1 or VgrG2b-Myc knockin ΔRetS PAO1 at a multiplicity of infection (MOI) of 30 and 20 μg/ml outer membrane vesicles (OMVs) for 2 hr.