GPR65 and neoplasm: To evaluate exhaustion and effector functions (29) in the tumor-infiltrating CAR T cells ex vivo (after restimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin), we first performed RNA-seq of isolated CAR T cells from mice without tumors or mice bearing m.CR or GPR65 KO tumors, followed by differential expression (DE) and pathway analysis.