Despite these differences, GPR65 KO tumor cells remained equally susceptible to CAR T cell–mediated lysis in vitro as m.CR tumors, even at low effector–to–target ratios, indicating that although scRNA-seq demonstrated nonidentity with m.CR tumors, GPR65 KO tumors are not intrinsically resistant to CAR T cell–mediated lysis. This evidence concerns the gene GPR65 and neoplasm.