Similar approaches with the use of antibiotin–AuNPs were developed for the simultaneous detection of 2 common SNPs (4 alleles) of the Toll-like receptor 4 (TLR4) gene related to the immune system [67] and the MBL2 gene [68], 10 SNPs in the HBA1 and HBA2 genes related to a-thalassemia using two strips that could detect 5 SNPs simultaneously and thus 10 alleles [69], and the Wilson disease-related ATP7B gene [70]. Here, TLR4 is linked to thalassemia.