PDGFRA and glioblastoma: In this study, we investigated the background effects of electroporation, lipofection, and recombinant adeno-associated virus (rAAV) transfection methods on the expression of PDGFRA and receptor tyrosine kinase (RTK) genes in human GBM cell lines U-251 MG and U-87 MG and human chronic myelogenous leukemia HAP1 cells to determine the optimal experimental conditions for genome editing.