Activation of AMPK, be it through exposure to salicylate, to the synthetic AMPK activator A76966 [39], phenformin (a biguanide), or through glucose deprivation, in all cases resulted in the reduction of c-Myc protein expression levels in U2OS osteosarcoma cells (Figure 3A). The gene discussed is PRKAA1; the disease is osteosarcoma.