To determine the potential role of MEOX2 in GBM initiation, Schonrock et al. differentiated hiPSCs to COs and, at the stage of cortical induction, these were nucleofected and genetically modified to suppress PTEN and TP53 expression by CRISPR/Cas9 and overexpress MEOX2, using a PiggyBac transposon system with the mNeonGreen reporter. Here, MEOX2 is linked to glioblastoma.