PGAM1 and cancer: While it is unknown whetherPGAM1-specific lysine methylation regulates PGAM1 function, PGAM1overexpression has been implicated in cancer and its dysregulationmay support oncogenic activity.53 The methodologiesemployed here provide a platform to first define and then prioritizepropargylation events for functional studies, such as examining therole of PGAM1 lysine methylation in the regulation of key steps inglycolysis.