RNA‐seq analysis and gene set enrichment analysis (GSEA) in TOMM20 overexpressing chondrosarcoma L2975 cells revealed suppression of apoptosis, and KEGG pathway analysis highlighted that ferroptosis, ubiquitin‐mediated proteolysis, and necroptosis were suppressed while the ECM‐receptor interaction pathway was activated. The gene discussed is TOMM20; the disease is chondrosarcoma.