It has demonstrated efficacy as an immunohistochemical biomarker of submucosal ganglion cells (Matsuda et al. 2006; Szabolcs et al. 1996), particularly useful in the exclusion of Hirschsprung disease (HSCR) by detecting ganglion cells and nerve fibrils more efficiently than biomarkers as MAP‐2 and Calretinine (Chisholm and Longacre 2016). This evidence concerns the gene MAP2 and Hirschsprung disease.