AIF1 and neoplasm: IBA1 is a frequently used marker ofthe phagocytic activity of brain macrophages; however, it can alsobe utilized for the quantification of macrophages in MPM tumors, giventhat AB12 tumor tissues were observed to be substantially infiltratedby IBA1+ macrophages.40 Biomarkerexpression was quantified using an automated QuPath algorithm to generatean H-score,41 which integrates both theintensity and distribution of the IHC staining.