To validate the sequencing results, we selected three genes (MUC1, MYO6, and IGKC) among the 11 important molecular marker candidates for silent transient cell line construction and transferred silent MUC1 and MYO6 into lung cancer cell line A-549, and silent IGKC into normal lung cell HPAEC. The gene discussed is MUC1; the disease is lung carcinoma.