In order to observe the effect of BAP31-ELAVL1-SPINK6 axis on HCC in vivo, we injected subcutaneously Balb/c nude mice with Hep3b cells under different treatments, measured the length, width and height of the tumor every 3 days, and removed the xenograft tumor 24 days after injection. This evidence concerns the gene SPINK6 and neoplasm.