What's more, western blot results showed that overexpression of SPINK6 could offset the increase of cell polarity marker and the decrease of interstitial marker caused by BAP31 or ELAVL1 knockdown, which further verified that BAP31-ELAVL1-SPINK6 axis induced cell polarity loss and promote EMT in HCC cells (Figure 6F). Here, BCAP31 is linked to hepatocellular carcinoma.