Therefore, our study aimed to comprehensively investigate the effects of high-dose ascorbate alone and in combination with paclitaxel on inhibiting cell proliferation and invasion in EC cells and a transgenic mouse model of endometrial cancer, including the impact of ascorbate on cellular stress and the PTEN/AKT/ mTOR pathway, as well as its underlying anti-neoplastic potential in EC treatment. Here, AKT1 is linked to endometrial cancer.