Furthermore, LUAD patients with deep tumour invasion and/or a large tumour size (T3/T4), a high American Joint Committee on Cancer (AJCC) 8th edition stage (stage III-IV) and lymph node metastasis (N1-N3) had higher expression of DNMT3a (Fig. 1c). This evidence concerns the gene DNMT3A and metastatic malignant neoplasm in the lymph nodes.