The results indicated that CHKA downregulation decreased the ECAR of Caco2 cells, and the addition of PCho significantly provided anaplerosis for the glycolysis in Caco2-shCHKA cells, suggesting that inhibition of the PI3K/AKT pathway could efficiently block the Warburg effect in tumor cells (Fig. 6F, G). Here, CHKA is linked to neoplasm.