Finally, when comparing the 24 week on diet data to the 8 week on diet data, we found that at 24-weeks there was: a lower proportion of Bmper+Nrxn1+ Fibroblasts in male rats fed a HF diet, a lower proportion of lymphatic ECs in male rats fed a control diet, a lower proportion of Piezo2+ lymphatic ECs in male rats fed a control diet, a greater proportion of arterial ECs in male rats fed either a HF or a control diet. Here, PIEZO2 is linked to hydrops fetalis.