CANX and glioma: Additionally, we assessed CANX expression in human normal astrocytes (NHAs), glioma cell lines (T98G, U118MG, A172, and LN229 cell lines), and primary GBM cells (GBM#P3, GBM#BG5, and GBM#BG7 cells) using Western blotting and qPCR (Figure 1M-O).