To corroborate these morphological and structural changes, H&E staining and Masson-trichrome staining were performed on the murine hearts, and the results indicated that MTAC minimally triggered the heart hypertrophic response in the absence of Nsun2 (Figure 4E, upper panel, H&E staining) but aggravated interstitial fibrosis of the Nsun2-devoid myocardium (Figure 4E, middle and lower panels, Masson staining), confirming the acceleration of heart failure progression. Here, NSUN2 is linked to heart failure.