CHRNA4 and peripheral arterial disease: While our previous studies in animal models have established a link between deterioration of NMJ morphology (motor nerve denervation and fragmentation of nAChR clusters) and functional abnormalities of the NMJ (decrease in endplate potentials) [21, 22, 24], the extent to which the observed problems in NMJ morphology in PAD patients with CLTI will impact the NMJ function is likely significant but needs further exploration.