To investigate the role of UFMylation in regulation of NLRP3 inflammasome activation, we first determined the expression of the core components (Ufl1, Ufm1, Ufc1, and DDRGK domain‐containing protein 1 (Ddrgk1)) in UFMylation cascade upon the NLRP3 inflammasome activation in immortalized bone marrow‐derived macrophages (iBMDMs), mouse BMDMs, and human myeloid leukemia mononuclear cells (THP‐1)‐derived macrophages. Here, NLRP3 is linked to myeloid leukemia.