To explore the clinical significance of the NAT10/CXCL2/KLF5/ITGαV axis in liver metastasis, we first examined the expression of NAT10, CXCL2, KLF5, and ITGαV in the primary tumor tissues of GC patients with liver metastasis (cohort 3), and the results revealed that NAT10 expression was positively correlated with CXCL2, KLF5, and ITGαV expression in the 23 GC patients with liver metastasis (R2 = 0.1997, p = 0.0326; R2 = 0.2862, p = 0.0085; and R2 = 0.2344, p = 0.0192, respectively). Here, KLF5 is linked to gastric cancer.