To investigate whether the disturbances observed in lipid metabolism in Gcgr−/− mice would be augmented when challenged with a diet high in cholesterol and fat, and to investigated if mice lacking glucagon receptor signaling were able to tolerate such a diet, we challenged female Gcgr−/− and Gcgr+/+ mice with a GAN diet (the Gubra Amylin Nonalcoholic steatohepatitis (GAN) diet consisting of 40 kcal% fat, 20 kcal% fructose, and 2% cholesterol) for 5 weeks and in separate experiments female Gcgr−/− and Gcgr+/+ mice were challenged with a HFD (58 kcal% fat and sucrose) for 8 weeks. This evidence concerns the gene GCGR and metabolic dysfunction-associated steatohepatitis.