TARDBP and amyotrophic lateral sclerosis: To overcome this limitation, we utilised brain tissue from the rNLS TDP-43 mouse model of ALS (NEFH-tTA/tetO-hTDP-43ΔNLS); the removal of dietary DOX induces the cytoplasmic accumulation of TDP-43 in neurons, causing motor neuron death in the spinal cord and motor cortex and associated motor phenotypes [62].