BRAF and neoplasm: Next-generation sequencing (NGS) was performed on the original tumor (block obtained from her home institution) and was negative for BRAF, KIT, NF1, and NRAS mutations, with homozygous loss of exon 3 of PTEN (chr10q), whole arm loss of chr6q, and detection of multiple coding variants, including both known pathogenic variants and variants of unknown significance (VUS) (Table 1).