As blebbisomes derived from breast cancer, glioblastoma and melanoma cells all contained CD47 (Extended Data Fig. 4c), and since we had noticed blebbisome expression of the immunosuppressive effector molecules CD73 (refs. 6,26) and Nectin-2/CD112 (ref. 43) (Supplementary Table 1 and Extended Data Fig. 4c), we wondered if cancer blebbisomes contain inhibitory immune checkpoint ligands6,44. This evidence concerns the gene NECTIN2 and melanoma.