Analysis by Western blotting showed the significant downregulation of CDC27 and O-GlcNAcylation protein in MM cells with gene knockdown of OGT. Consistently, the upregulation of CDC27 and O-GlcNAcylation protein levels was observed in Thiamet G-treated MM cells (Fig. 2d). This evidence concerns the gene OGT and Miyoshi myopathy.