Lin et al. [109] established a zebrafish model of ICC and discovered that fish oil rich in ω-3 PUFAs not only suppressed the migration and invasion ability of CCA cells by downregulating the expression of MMP7, MMP9, TWIST, Snail-1, and VEGFA but also disrupted the G2/M phase of the cell cycle and suppressed the proliferation of CCA cells. This evidence concerns the gene VEGFA and cholangiocarcinoma.