To explore whether the efficacy of ICT in OS is related to different TMEs associated with MTAP status, we further utilized the single-cell transcriptomic dataset (n = 12) from our clinical cohort to elucidate the OS TME with different MTAP statuses, including 6 metastases, 2 primary tumor lesions, and 4 online data (Table S2; Figure S1A). This evidence concerns the gene MTAP and neoplasm.