ZBP1 and infection: Activation markers of ZBP1-mediated necroptosis, including RIPK3 phosphorylation (pRIPK3) and MLKL phosphorylation (pMLKL), were attenuated in R1-ICR-3–treated HT29-hZBP1 cells during HSV-1(ICP6mut) infection (Fig 3B).