KRAS and infection: Figure 2B shows a representative immunoblot of this experiment, while the graph in Figure 2C summarizes multiple repeats. NS3 expression was undetectable when NH4Cl was added 30 min before, together with, or 30 min following EHDV2-Ibaraki challenge. The addition of NH4Cl at later time points (up to 2 h) resulted in progressive increase in infection in multiple experiments, while a lesser increase was observed between the 2 or 3-hour time point of NH4Cl addition. These results suggest that the critical step inhibited by NH4Cl occurs within the initial 2-3 hours of infection.