In vitro knockdown of 6PGD or pharmacological inhibition significantly suppressed HCC growth and promoted apoptosis, potentially mediated by the activation of the AMPK pathway, which increases acetyl-CoA carboxylase 1 (ACC1) activity and decreases SIRT1 activity, thereby affecting lipid synthesis and other metabolic changes mediated by SIRT1. The gene discussed is SIRT1; the disease is hepatocellular carcinoma.