Coactivator-associated arginine methyltransferase 1 (CARM1 or PRMT4) can reduce GAPDH’s affinity for its coenzyme NAD+ by methylating arginine 234 (R234) in GAPDH, thereby inhibiting GAPDH activity, further inhibiting glycolysis, and suppressing the growth and proliferation of HCC. This evidence concerns the gene GAPDH and hepatocellular carcinoma.