Specifically, our study included mouse genotype as a variable to assess how mucosal washes perform under conditions that resemble human disease, such as IBD.32 This study does not aim to determine the precise extent of the effect of TLR5 deficiency or Crohn’s disease (CD) on microbiota differences, nor can this be separated from other confounding factors such as housing conditions in our current experimental design. This evidence concerns the gene TLR5 and Cowden disease.