DSTYK knockdown also inhibited the phosphorylation of TBK1, IRF3, STING, P65 and IκBα following pDNA transfection and infection with vaccinia virus (VACV; a DNA virus; Fig. EV3A,B,D,E,G); however, SeV-induced responses were not altered (Fig. EV3C,F,H). This evidence concerns the gene IRF3 and infection.