To test whether autophagy inhibition triggered by HHT in MLKL-deficient cells is the cause of CRC cell loss, we treated the MLKL KO cells with HHT and the cell permeable peptide composed of the HIV-1 TAT protein transduction domain attached to either a scrambled amino acid sequence (control peptide) or the peptide derived from a protein Beclin-1, a major autophagy inducer that promotes autophagy by activating class III PI3 kinase (PI3K) [44]. The gene discussed is MLKL; the disease is colorectal carcinoma.