PRNP and prion disease: Both infected mice developed clinical prion disease (S4 Table and Fig 10B) and propagated diglycosylated PrP dominant PrPSc (detectable by direct western blotting) and patterns of PrP deposition by IHC mirroring that seen after transmission of either 129MM vCJD or 129MV vCJD patient brain samples in these mice.