In 129MV Tg35c/152c mice, 13/15 inoculated recipients were infected (of which two developed clinical prion disease) and all infected mice propagated type 5 PrPSc accompanied by the typical neuropathology associated with this molecular strain type (prominent, irregular non-florid PrP plaques in the corpus callosum) (S2 Table and Figs 1 and 2). The gene discussed is PRNP; the disease is prion disease.