APC and colorectal adenoma: We recruited a cohort of patients with sporadic colorectal adenoma and performed targeted sequencing on the APC gene along with in situ quantitative detection of phosphor-YAPS127 (p-YAP), N-terminal APC protein (APCn), and other key regulatory molecules of Wnt pathway (β-catenin) [23], G-protein signaling (Asef1/2) [24,25], and APC-correlated transcriptional coregulators (i.e. C-terminal binding protein (CtBP) and microtubule-associated protein RP/EB family member 1 (EB1)) [26–28].