In contrast, four cases with RUNX1 fused to other genes (EVX1, POU2F2, USP42, and ZEB2) neither cluster with the RUNX1::RUNX1T1 group nor form a distinct cluster in gene expression profiling (Supplementary figure S10B), suggesting that different biologic mechanisms may be associated with these rare RUNX1 fusions in AML, and their clinical significance remains unclear. This evidence concerns the gene POU2F2 and acute myeloid leukemia.