NUSAP1 and central nervous system cancer: Based on inferred copy number variations (CNV), cells with elevated CNV levels were classified as glioma cells (Supplementary Figure 1) revealing three subpopulations: C0 MALAT1+ glioma cells (2,508), C1 AKAP9+ glioma cells (1,788), and C2 NUSAP1+ glioma cells (1,566) (Figure 2A).