The aims of this study were: 1) to verify whether IFNβ promotes ferroptosis in tumor cell lines; 2) to investigate which proteins play essential roles in IFNβ-mediated ferroptosis; 3) to observe whether IFNβ can synergize with classical ferroptosis inducer RSL3 to exert anti-tumor effects. The gene discussed is IFNB1; the disease is neoplasm.