To help resolve this valid scientific concern we did a study in which we compared infection with Leptospira interrogans serovar Copenhageni Fiocruz in mice expressing fully competent tlr4 (C3H-HeN, C57BL6) versus tlr4 hyporesponsive mice (C3H-HeJ) over a period of two weeks. This evidence concerns the gene TLR4 and infection.