Specifically, TDP‐43 interaction with ALKBH5 facilitates ALKBH5 nuclear translocation and thus its binding to CDC25A pre‐mRNA in fast‐growing GBM cells.TDP‐43/ALKBH5 complex demethylates CDC25A pre‐mRNA, inhibiting its exon 6 skipping and preserving the expression of the oncogenic isoform CDC25A‐1, thereby promoting G1/S phase transition and growth of GBM cells. Here, TARDBP is linked to glioblastoma.