Overall, P/LP CPG variants were identified in 23.3% (193/830) of patients, including 30 with two variants and two with three variants: one patient with HGG and three MSH6 P/LP variants and another with subependymal giant cell astrocytoma (SEGA, PT_JW6FBEFK) that harbored TSC2, ASXL1, and APC P/LP variants (Table S4A). The gene discussed is TSC2; the disease is astrocytoma (excluding glioblastoma).