To investigate the morphological patterns underlying the prediction of genetic alterations in CRC from H&E slides using DL, we analyzed WSI heatmaps (Fig. 5, Fig. S6–S10) and highly predictive tiles (‘top tiles’, Fig. 6, Fig. S11–S23) for key prediction targets (Cluster 1: TP53, APC, KRAS; Cluster 2: RNF43, BRAF, hypermutated) in 25 cases from CRA and WHI test cohorts. The gene discussed is APC; the disease is colorectal carcinoma.