Frequent MSI-associated patterns in most MSI cases and Cluster 2 gene mutations included medullary growth, high tumor-infiltrating lymphocytes (TILs), and mucinous differentiation, consistent with prior studies.37–39 However, some MSI cases in our cohort and the literature display atypical morphologies not commonly linked to MSI (e.g., Fig. S8D, Fig. S9D).40 In MSS-related Cluster 1, KRAS mutation predictions highlighted luminal tumor regions, especially villous adenomas with high-grade dysplasia, as equally important as invasive adenocarcinoma areas (Fig. S16A–B, Fig. S17A–B). This evidence concerns the gene KRAS and neoplasm.