From cocultures containing equal numbers of targets and effectors across T-cell conditions, we examined >20,000 conjugates at 15, 30, and 60 minutes and assessed the dynamics of CPR41BB recruitment to the CARIS (Fig. 5C) with both WT (PD-L1+/PD-L2+) and PD-L1 KO (PD-L1−/PD-L2+) LN229-GBM cells (Supplementary Fig. S7A and S7B). Here, PDCD1LG2 is linked to glioblastoma.