When compared across CART, F-actin polarization in CARζ/CPR41BB conjugates was higher compared with first-generation CARζ cells in both WT and PD-L1 KO LN229-GBM conditions by 60 minutes (**, P < 0.01; ****, P < 0.0001, two-way ANOVA with the Tukey test; Fig. 5F), whereas actin enrichment at the synapse interface was similar between CARζ/CPR41BB and second-generation CAR41BBζ cells (P = 0.826, two-way ANOVA with the Tukey test). Here, CD274 is linked to glioblastoma.