In the present study, by constructing a series of luciferase reporter plasmids containing various regions of 3’-UTR of PRNP, analyzing the luciferase activities in both CL1-1 (low invasiveness) and CL1-5 (highly invasiveness) lung cancer cells, we identified four potential PRNP targeting miRNAs that differentially expressed in CL1-1 and CL1-5 (Fig. 1E). This evidence concerns the gene PRNP and lung cancer.