Histochemical analysis based on H&E and Oil red O staining revealed that Enpp1 overexpression reduced hepatic steatosis (Fig. 4F), which was consistent with the reduction in liver TG levels, liver T-CHO levels and serum TG levels in Enpp1-overexpressed HFD-fed mice (Fig. 4G-I). The gene discussed is ENPP1; the disease is fatty liver disease.