GO enrichment analysis indicated associations with steroid and small molecule metabolism (Fig. 6B), while KEGG pathway analysis identified multiple enriched pathways, including Th17 cell differentiation, PPAR signaling pathway, PI3K-AKT signaling pathway, cancer-related pathways, mTOR signaling pathway, metabolic pathways, MAPK signaling pathway, HIPPO signaling pathway, HCC, focal adhesion, and apoptosis signaling pathway (Fig. 6C). The gene discussed is AKT1; the disease is cancer.