Notably, the levels of MIR181A1HG in the CM of CRC cells remained unchanged upon RNase A treatment but significantly decreased following treatment with RNase A plus Triton X-100 (Supplementary Fig. S2e), which indicated that extracellular MIR181A1HG was primarily encapsulated within a membrane rather than directly released. Here, RNASE1 is linked to colorectal carcinoma.