HNRNPR and colorectal carcinoma: As EV-mediated transfer of RNAs requires a specific and selective RNA-binding protein for transportation [17], to assess the specific interaction between the MIR181A1HG sequence and the specific RNA-binding proteins regulating MIR181A1HG packaging into EVs, we used RNA pull-down combined with mass spectrometry analysis and found that HNRNPA2B1, IGF2BP2, and HNRNPR could bind to MIR181A1HG in CRC cells (Fig. 3a).