As EV-mediated transfer of RNAs requires a specific and selective RNA-binding protein for transportation [17], to assess the specific interaction between the MIR181A1HG sequence and the specific RNA-binding proteins regulating MIR181A1HG packaging into EVs, we used RNA pull-down combined with mass spectrometry analysis and found that HNRNPA2B1, IGF2BP2, and HNRNPR could bind to MIR181A1HG in CRC cells (Fig. 3a). This evidence concerns the gene HNRNPA2B1 and colorectal carcinoma.