Furthermore, following transient transfection with Cy3 (red)-tagged MIR181A1HG, CRC cell-derived EVs were cocultured with LX2 cells, and fluorescently labeled MIR181A1HG was detected in LX2 cells (Fig. 2j), suggesting that MIR181A1HG was transferred from CRC cells to HSCs via EVs, with a greater quantity of MIR181A1HG transferred from highly metastatic CRC cells. This evidence concerns the gene MIR181A1HG and colorectal carcinoma.